Validation · ATAC-seq
ATAC-seq across melanoma cell lines, ~20 samples
Differential accessibility between melanoma states should recover MITF and SOX10 motifs in differentially-accessible regions — these are the master regulators of the melanocyte lineage and the most-replicated melanoma transcription-factor signal.
DiffBind PCA cleanly separates conditions on PC1. Motif analysis in differentially-accessible peaks recovers MITF and SOX10 as top-enriched motifs. ChIPseeker peak annotation matches expected promoter/enhancer distribution.
ATAC-seq pipeline benchmark covering peak calling (MACS3), QC (TSS enrichment, fragment-size distribution), differential accessibility (DiffBind), and downstream motif analysis. Recovers the expected lineage-defining transcription factors as top-enriched motifs.
Same pipeline, fixed-fee, 7–10 business days. Email with your omics type and sample count.